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. 2020 Apr 30;10:7396. doi: 10.1038/s41598-020-63998-2

Figure 1.

Figure 1

Study design. BALB/c mice (20 per group) were immunized with 4 µg outer membrane vesicle pertussis vaccine (omvPV) by the intranasal (IN; red) or subcutaneous (SC; blue) route on days 0 and 28. Subsequently, for both routes the vaccination-induced responses were characterized at day 35 and 56 (n = 6). Subsequently, a B. pertussis challenge (2 × 105 colony-forming units (CFUs)) was performed on day 56 in both vaccinated groups and naive mice (green) after which the level of protection was assessed in the respiratory tract on day 57 and 62 (n = 4).