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. 2020 Apr 24;10:147. doi: 10.3389/fcimb.2020.00147

Figure 1.

Figure 1

The criteria program and an illustration of the UPT-LF assay. (A) Program for the UPT-LF assay criteria. (B) The photograph of UPT biosensor scanning of a strip. (C) The detection result shows the concentration of bacteria. The signal peak on the left is derived from the control band, and that on the right is derived from the test band. (D) Information stored by the UPT-LF biosensor including time, sample ID, detection target, concentration of the target, the value of the signal for the test band and control band, T/C ratio, as well as the parameters for standard curves (y = Ax + B, the logarithm of T/C-cutoff as x and the logarithm of concentration as y) for quantitation of the concentration of target bacteria, including the cut-off, A and B. Some information, with the exception of the detection result, was recorded in radiofrequency identification devices (RFID) for each commercial strip, and this can be revised through a new record after calibration.