U0126 blocked the activation of ERK1/2 signaling pathway in Müller cells. After stimulated by the serum from different groups of blood-stasis syndrome rats, U0126 was added into the culture medium of Müller cells and incubated for 24 h. Subsequently, western blot analysis was conducted to detect the expression levels of p-ERK1/2 and ERK1/2. The data were expressed as mean ± SD, P value less than 0.05 was considered statistically significant, *P < 0.05, **P < 0.01, ***P < 0.001