Fig. 3.

Effect of LA on HUVEC injury. a HUVECs were incubated with LA (500 μM) for 48 h. LDH activity in cells and culture medium were examined. The leakage of LDH was expressed as the percentage of medium LDH activity over total activities. n = 3/group. LA, α-lipoic acid; LDH, lactic acid dehydrogenase. b Following treatment with LA (500 μM) for 48 h, Hoechst33342 was stained in the HUVECs to examine nuclear condensation. n = 3/group. LA, α-lipoic acid