Fig. 7.

Overexpression of HSPA12B attenuated the inhibitory effects of LA on VEGF expression. HUVECs were infected with HSPA12B adenovirus (Ad-HSPA12B) or empty adenovirus (Ad-GFP). The scheme for adenovirus construction was shown (a). Twenty-four hours after infection, cells were incubated with LA (500 μM) for 48 h. Untreated cells served as controls (Con). After experiments, HUVECs were harvested for immunoblotting analysis against HSPA12B (b) or VEGF (c). The blots for α-tubulin served as loading controls. **P < 0.01 and *P < 0.05, n = 3–9/group