Table 4.
Preclinical models of BsAbs targeting MM.
| Targets | Drug | Design | Model | References |
|---|---|---|---|---|
| BCMA × CD3 | EM801 | IgG1 Fc region | MM cell and effector cell co-cultures BMAs of MM patients (autologous T cells) NOG mice with human myeloma allogeneic xenograft Cynomolgus monkeys | Seckinger et al. (85) |
| BCMA × CD16A | AFM26 | Tetravalent, Fc region | NK cell cultures, serum IgG MM cell and primary human NK cell co-cultures MM cell and PBMC co-cultures | Gantke et al. (62) |
| BCMA × NKp30 | CTX-8573 | IgG1 Fc region | MM and NK cell co-cultures, in the presence of sBCMA, sBAFF and sAPRIL Humanized mice models engrafted with MM tumors Cynomolgus monkeys | Watkins-Yoon et al. (15) |
| BCMA × CD3 | AP163 | Information not available | MM and effector cell co-cultures NSG mice models with human PBMCs and MM or Burkitt lymphoma tumor cells | Li et al. (16) |
| CD138 × CD3 | STL001 or BiTE-hIgFc | scFvs and IgG1 Fc region | MM cell and PBMC co-cultures T cell activation assay NSG mice with human myeloma xenograft | Zou et al. (17) |
| CD138 × CD3 | H-STL002 & M-STL002 | scFvs and IgG1 Fc region | MM cell and PBMC co-cultures | Chen et al. (86) |
| CD38 × CD3 | Sorrento CD38/CD3 | scFv-Fc region fusion chain and Fab arm | MM cell and PBMC co-cultures NSG mice models with implanted MM or Burkitt lymphoma tumor cells and unstimulated PBMCs | He (87) |
| CD319 × NKG2D | CS1-NKG2D | BiKE | IL-2 primed NK cultures IL-2 primed PBMC with high, intermediate, low CS1 expression MM cell line co-cultures NSG mice engrafted with human PBMCs and high- and intermediate-CS1 expressing MM cell line xenografts | Chan et al. (19) |
| GPRC5D × CD3 | GPRC5D TRAB | IgG region | MM cell and unstimulated PBMC co-cultures NSG mice model inoculated with human T cells and MM tumor cells NOG mice model engrafted with CD34+ hematopoietic stem cells and MM tumor cells | Kodama et al. (88) |
| NY-ESO-1 × CD3 | ImmTAC-NYE | TCR-like HLA-A2/NY-ESO-1157−165arm, scFv, peptide linker | MM cell and CD8+ cell co-cultures | McCormack et al. (89) |
| NY-ESO-1 × CD3 | A2/NY-ESO-1157 –specific BsAb | anti-HLA-A2/NY-ESO-1157−165 scFv, scFv, peptide linker | Peripheral blood T cells and T2 cells loaded with NY-ESO-1157 peptide co-cultures MM cell and peripheral blood T cell co-cultures Peripheral blood T cells and cells presenting the NY-ESO-1157−165 peptide by HLA-A*02:06 co-cultures NOG mice model engrafted with MM cells and activated T cells | Maruta et al. (69) |