TABLE 2.
Regio- and Stereo-selective NHase reported in recent years.
| Origin and (Potential application) | Substrate | Regio-/stereo-selectivity | Conversion rate | Main product | References |
| Pseudomonas putida NRRL-18668 (Bioproduction of pharmaceuticals and agricultural chemicals) | Adiponitrile/malononitrile | Regioselectivity | 99.5% (adiponitrile) | 5-cyanovaleramide (95.7%) cyanoacetamide (97.8%) | Cheng et al., 2016 |
| Pseudomonas putida NRRL-18668 βL37Y mutant (Bioproduction of pharmaceuticals) | Adiponitrile/malononitrile | Regioselectivity | 98.8% (adiponitrile) | Adipoamide (96.1%) Malomamide (97.2%) | Cheng et al., 2016 |
| Comamonas testosteroni 5-MGAM-4D (Bioproduction of pharmaceuticals) | Adiponitrile/malononitrile | Regioselectivity | 100% (adiponitrile) | Adipoamide (97.9%) Malomamide (98.2%) | Cheng et al., 2016 |
| Comamonas testosteroni 5-MGAM-4D βF37P mutant (Bioproduction of pharmaceuticals and agricultural chemicals) | Adiponitrile/malononitrile | Regioselectivity | 94.1% (adiponitrile) | 5-cyanovaleramide (90.4%) cyanoacetamide (96.6%) | Cheng et al., 2016 |
| R. aetherivorans ZJB1208 (Bioproduction of pharmaceuticals and agricultural chemicals) | 1-cyanocyclohexaneacetonitrile | Regioselectivity | 100% | 1-cyanocyclohexaneacetamide (966.7g/L) | Zheng et al., 2016 |
| Rhodococcus ruber CGMCC3090 (Bioproduction of agricultural chemicals) | Adiponitrile | Regioselectivity | 100% | 5-cyanovaleramide (99.2%) | Shen et al., 2012b |
| Rhodococcus rhodochrous J1 (Bioproduction of pharmaceuticals) | Adiponitrile/malononitrile/terephthalonitrile/phthalodinitrile | Regioselectivity | 98.6% (adiponitrile) 97.3% (malononitrile) 99.2%(terephthalonitrile) 96.1%(phthalodinitrile) |
Adipamide (100%) Malomamide (77.3%) Terephthalamide (84.3%) Phthalamide (100%) | Cheng et al., 2018a |
| Rhodococcus rhodochrous J1 βY68T/W72Y mutant (Bioproduction of pharmaceuticals and agricultural chemicals) | Adiponitrile/malononitrile/terephthalonitrile/phthalodinitrile | Regioselectivity | 70.5% (adiponitrile) 79.5% (malononitrile) 71.1% (terephthalonitrile) 72.5% (phthalodinitrile) | 5-cyanovaleramide (100%) cyanoacetamide (97.1%) 4-cyanobenzamide (98.2%) 2-cyanobenzamide (100%) | Cheng et al., 2018a |
| Rhodopseudomonas palustrisHaA2 (Bioproduction of pharmaceuticals) | 2-phenylpropionitrile/2-Phenylbutyronitrile | Stereoselectivity | Not available | S-product (E-value > 100)/ S-product (E-value 53) | van Pelt et al., 2011 |
| Rhodopseudomonas palustrisCGA009 (Bioproduction of pharmaceuticals) | 2-phenylpropionitrile/2-Phenylbutyronitrile | Stereoselectivity | Not available | S-product (E-value > 100)/ S-product (E-value 95) | van Pelt et al., 2011 |
| Rhodococcus sp. AJ270 (Bioproduction of pharmaceuticals) | 2-phenylbutyronitrile/3-Benzoyloxypentanedinitrile/Naproxennitrile | Stereoselectivity | Not available | R-product (ee 83%)/ S-product (ee 68.2%)/ S-product (E-value 80) | Wang, 2005; Song et al., 2007; van Pelt et al., 2011 |
| Rhodococcus rhodochrous J1 (Bioproduction of pharmaceuticals) | rac-mandelonitrile | Stereoselectivity | 87.3% | S-product (ee 52.6%) | Cheng et al., 2018b |
| Rhodococcus rhodochrous J1 βF37H mutant (Bioproduction of pharmaceuticals) | rac-mandelonitrile | Stereoselectivity | 80.1% | S-product (ee 96.8%) | Cheng et al., 2018b |