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. 2020 Apr 24;11:535. doi: 10.3389/fmicb.2020.00535

TABLE 6.

Acid-evolved clone J3-1 mutations.*

Replicon Start bp End bp Mutation Annotation Gene description
Chromosome 749,943 749,954 (11 bp)1→2 ISH: coding (562/2007 nt) [vng0985H→]
Chromosome 1,089,129 1,089,137 (8 bp)1→2 ISH: coding (15/789 nt) [vng1467G→] bop (bacterio-opsin).
Chromosome 1,163,363 A→G K197R (AAA→AGA) [vng1561→] Ferredoxin
Chromosome 1,229,749 1,229,760 (11 bp)1→2 ISH: coding (582/849 nt) In vng1650
PNRC100 0 7,788 Δ7788 bp ISH7-1 deleted [vng7001←] – [←vng_RS13745]
PNRC100 71,210 74,656 Δ3447 bp ISH2 mediated (vng RS13790→) – [←vng7073] SOS response peptidase (deleted)
PNRC100 133,743 142,521 Δ8779 bp ISH8-2, 3-3 mediated (vng RS12330←) – (→vng7127) Arsenic resistance operon (deleted)
PNRC100 143,909 150,253 Δ6345 bp ISH3-3, 2 mediated (vng RS12880→) – [→vng7136] ssDNA-binding protein A (deleted)
PNRC200 0 7,760 Δ7760 bp ISH 7-1 deleted (vng6001H←) – [←vng6011H]
PNRC200 71,219 74,595 Δ3377 bp ISH 2 mediated (vng6094H→) – [←vng6097C] SOS response peptidase (deleted)
PNRC200 244,422 244,430 9 bp(1→2) ISH: intergenic (vng6313G←) / (vng6315G←) arcD Arginine:ornithine antiporter/ornithine carbamoyItransferase
PNRC200 249,147 249,157 11 bp(1→2) ISH: coding [vng6318G←] arcR Arginine catabolism transcription a I regulator
PNRC200 262,603 265,437 Δ2835 bp ISH 3-2, 8-3 mediated/ISH 8-3 deleted (vng_RS11685←) / (→vng6331H)
PNRC200 309,256 309,812 Δ557 bp ISH 2, 8-3 mediated (vng6393H←) – (←vng6395H)
PNRC200 311,213 323,320 Δ12108bp ISH 8-3, 11 mediated (vng6396H→) – (←vng6420H) arsR transcriptional regulator, phzF phenazine biosynthesis
PNRC200 324,386 332,792 Δ8407 bp ISH 11 mediated (vng_RS12000→) – (→vng6441H)

* “Annotation” column code: “ISH *** mediated” = flanking ISH elements, missense mutations in blue. “Gene” column code: (mutation starts or ends before this gene name), → ← indicates gene directionality, [mutation starts, ends, or is entirely contained within this gene name], “-” indicates intervening omitted genes found in description, “/” indicates mutation is between two genes. Highlight indicates mutations associated with acid evolution.