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. 2020 Apr 24;14:96. doi: 10.3389/fncel.2020.00096

FIGURE 13.

FIGURE 13

Effect of Src/P115-RhoGEF/ROCK signaling pathway on tumor cells TEM. (A) After pre-treating with Y-27632 (10 μM) or Bosutinib (2 μM) for 1 h, VMECs were treated with CX3CL1 (1 μg/ml) for 6h, and the TEM was determined using fluorescently labeled cancer cells (7860-O-mCherry and A-549-GFP) in the Transwell chamber. (B) The TEM rate of fluorescently labeled cancer cells was presented as a histogram. *P < 0.05 vs. the corresponding CX3CL1. (C) After transfection with P115-RhoGEF siRNA for 48 h, VMECs were treated with CX3CL1 (1 μg/ml) for 6 h, and the TEM was determined using fluorescently labeled cancer cells (7860-O-mCherry and A-549-GFP) in the Transwell chamber. (D) The TEM rate of fluorescently labeled cancer cells was presented as a histogram. *P < 0.05 vs. the corresponding control siRNA group.