Fig. 1.

Inhibition of PBK expression or activity promotes paclitaxel‐induced H460 cell death. (A) H460 cells were treated with DMSO and 0.1 μg·mL⁻¹ of paclitaxel alone or together with HI‐TOPK 032 (3 μm) for indicated time, or were transfected with control siRNA or PBK siRNA, and then incubated with paclitaxel. Cell viability was measured by WST‐1 cell proliferation assay. (B) Control siRNA cells (Con) or stable PBK siRNA cells (1, 2, 5 clones) were established using G418. Immunoblotting was done to verify PBK expression. (C) Control siRNA cells or stable PBK siRNA cells were treated with DMSO or paclitaxel (0.1 μg·mL⁻¹) for 12 or 24 h. Immunoblot analysis was performed using PBK or cleaved PARP antibody. (D) Control siRNA cells or stable PBK siRNA cells were incubated with DMSO or paclitaxel (0.1 μg·mL⁻¹) for indicated time. Cell lysates were subjected to immunoblotting using indicated antibody. Endogenous β‐actin level was used for loading control. Representatives of three independent experiments are shown. *P < 0.05; **P < 0.01; and ***P < 0.001 compared to controls. Statistical analysis was done by two‐tailed Student's t‐test.