Fig. 4.

PBK is required for paclitaxel‐induced p53 ubiquitination. (A) 293 cells were transfected with GFP‐p53 together with HA‐Ub construct and were incubated with DMSO or paclitaxel (0.1 μg·mL⁻¹) at 24 h post‐transfection for indicated time. Cell lysate was subjected to immunoprecipitation using GFP antibody and protein A/G plus‐agarose bead, and then, immunoblotting was done using HA antibody. Also, cell lysate was probed with indicated antibody. (B) Control siRNA cells or PBK siRNA cells were transfected with GFP‐p53 plus HA‐ubiquitin (Ub) construct and were treated with DMSO or paclitaxel (0.1 μg·mL⁻¹) 24 h after transfection for indicated time. Immunoprecipitation using GFP antibody and subsequent immunoblotting using HA antibody was performed. Cell lysate was subjected to immunoblotting using indicated antibody. Shown are representatives of three independent experiments.