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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Reproduction. 2020 Jun;159(6):659–668. doi: 10.1530/REP-19-0436

Figure 4. Reduced proliferation and increased apoptosis in Fancd2-KO PGCs.

Figure 4.

(A) Reduced proliferation in Fancd2-KO PGCs. GFP+ PGCs from E11.5 embryos of WT and Fancd2−/− females expressing the Pou5f1-eGFP reporter gene were sorted by FACS and cultured on STO feeder cells for 15 days. Images were taken at the indicated time points. (B) Cumulative growth curve of PGCs during PGC culture initiation. GFP+ PGCs from E11.5 embryos of WT and Fancd2−/− females expressing the Pou5f1-eGFP reporter gene were sorted by FACS and cultured on STO feeder cells for the indicated days. Values indicate mean ± SD of three independent experiments. *p < 0.05, **p < 0.01. (C) Diminished BrdU incorporation in Fancd2-KO PGCs. GFP+ PGCs from E11.5 embryos of WT and Fancd2−/− females expressing the Pou5f1-eGFP reporter gene were sorted by FACS and subjected to pulse-labeling with BrdU followed by flow cytometric analysis for BrdU-positive cells. Values indicate mean ± SD of three independent experiments. *p < 0.05. (D) Increased apoptosis in Fancd2-KO PGCs. GFP+ PGCs from E11.5 embryos of WT and Fancd2−/− females expressing the Pou5f1-eGFP reporter gene were subjected to flow cytometric analysis for Annexin V-positive, DAPI-negative apoptotic cells. Results are presented as mean ± SD of three independent experiments. **p < 0.01