Fig. 2.

Pannus formation, bone destruction, and ankylosis in bColII-D1BC mouse [16]. a–d H&E staining for pannus formation and bone destruction (a, b) and ankylosis (c, d) in bColII-D1BC mouse joints. D1BC mice were injected with a low dose of bColII (0.02 mg/mouse). Following chondrogenesis, hypertrophic chondrocytes (HCs) were localized adjacent to invasive synovial fibroblasts in pannus in D1BC mouse joints. e and f Immunohistochemical staining of vimentin (red), S100A4 (yellow) ,and Mac3 (green) in bColII-D1BC mouse joints. Each box in a, c, and e represents a magnified area from each right panel. Scale bar = 50 μm