Fig. 3.

(A) Confocal microscope images of Caco-2 cells incubated with LC-PLGA NPs at 37 °C for 1 h. Blue: DAPI for nuclei, Green: coumarin 6. (B) The effects of the substrate/ligand density on the nanoparticle absorption. Adapted from Kou et al. [83] (C) Confocal microscope images and fluorescence intensity for flow cytometry of Hela cells incubated with liposomes for 4 h at 37 °C (left or blue) and 42 °C (right or red). Nuclei were stained blue (DAPI), CF appeared green, and lysosomes were stained red (LysoTracker Red DND-99). (D) Schematic illustration showing the LAT1-targeting thermoresponsive liposomes. Adapted from Maekawa-Matsuura et al. [90].