Table 1A.
Human primer sequences used for real-time quantitative RT-PCR.
| Gene name | Orientation | Sequence |
|---|---|---|
| SLC7A11 | Forward | TGG ACG GTG TGT GGG GTC CT |
| SLC7A11 | Reverse | CAG CAG TAG CTG CAG GGC GTA |
| p53 | Forward | GGA GCA CTA AGC GAG CAC TG |
| p53 | Reverse | TCT CGG AAC ATC TCG AAG CG |
| p21 | Forward | TTT CCC TTC AGT ACC CTC TC |
| p21 | Reverse | CTT CTT CTT GTG TGT CCC TTC |
| BAX | Forward | CGG AAC TGA TCA GAA CCA TC |
| BAX | Reverse | CTG GAG ACA GGG ACA TCA |
| Cyclin D1 | Forward | CAC TCC TAC GAT ACG CTA CTA |
| Cyclin D1 | Reverse | CAT CTC ATA AAC AGG TCA CTA CA |
| Vimentin | Forward | CCA GCT AAC CAA CGA CAA A |
| Vimentin | Reverse | TCC TCT CTC TGA AGC ATC TC |
| E-cadherin | Forward | CTC CCT TCA CAG CAG AAC TAA C |
| E-cadherin | Reverse | CCA CCT CTA AGG CCA TCT TTG |
| Zeb1 | Forward | AAG TGG CGG TAG ATG GTA ATG |
| Zeb1 | Reverse | AGG AAG ACT GAT GGC TGA AAT AA |
| Zeb2 | Forward | GAG AAA GTA CCA GCG GAA ACA |
| Zeb2 | Reverse | AGG AGT CGG AGT CTG TCA TAT C |
| Snail | Forward | CCA CGA GGT GTG ACT AAC TAT G |
| Snail | Reverse | ACC AAA CAG GAG GCT GAA ATA |
| Twist | Forward | CGG AGA CCT AGA TGT CAT TGT TT |
| Twist | Reverse | ACG CCC TGT TTC TTT GAA TTT G |
| HPRT | Forward | GCG TCG TGA TTA GCG ATG ATG AAC |
| HPRT | Reverse | CCT CCC ATC TCC TTC ATG ACA TCT |