Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 29;105(5):1317–1328. doi: 10.3324/haematol.2019.217687

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright© 2020 Ferrata Storti Foundation

Material published in Haematologica is covered by copyright. All rights are reserved to the Ferrata Storti Foundation. Use of published material is allowed under the following terms and conditions:

https://creativecommons.org/licenses/by-nc/4.0/legalcode. Copies of published material are allowed for personal or internal use. Sharing published material for non-commercial purposes is subject to the following conditions:

https://creativecommons.org/licenses/by-nc/4.0/legalcode, sect. 3. Reproducing and sharing published material for commercial purposes is not allowed without permission in writing from the publisher.

PMC Copyright notice

Molecular profiling of PDTALL8 control and OMP52M51 resistant T-cell acute lymphoblastic leukemia cells. (A) Comparison between the average mutational burden in PDTALL8 untreated and resistant cells based on whole exome sequencing (WES) results. Total: total number of single-nucleotide variant (SNV); filtered: variants with a variant allele fraction (VAF) >10% and coverage depth (DP) >30 excluding common polymorphisms; coding: filtered coding variants excluding synonymous genetic alterations. (B) Venn diagrams of coding variants for each mouse. 440 SNV and 54 SNV are shared respectively between control (#1-3) and resistant mice (#5-7) inside each group. (C) NOTCH1 variants found only in OMP52M51 resistant samples. cDNA coordinates, amino acidic changes, VAF, alternative allele depth (AD) and DP are reported. (D) Direct sequencing of exon 26 in a representative control (Ctrl Ab #1) and resistant (OMP52M51 resistant #5) pair. Resistance-related mutations are indicated with the red arrows. (E) Flow cytometric analysis of surface expression of NOTCH1 in T-cell acute lymphoblastic leukemia (T-ALL) cells from the spleen of PDTALL8 mice treated with either OMP52M51 or control antibody. Two representative samples per group are shown.