| Protein concentration (mg ml−1) |
9.0 |
11.0 |
11.0 |
14.0 |
| Buffer composition of protein solution |
10 mM Tris–HCl pH 8.0, 10 mM CaCl2
|
10 mM Tris–HCl pH 8.0, 10 mM CaCl2, 2 mM FV peptide (929–937, SDLLLLKQS) |
10 mM Tris–HCl pH 8.0, 10 mM CaCl2, 2 mM FV peptide (929–937, SDLLLLKQS) |
10 mM Tris–HCl pH 8.0, 10 mM CaCl2,, 2.5 mM FVIII peptide (776–816) |
| Composition of reservoir solution |
1.2 M sodium malonate, 0.5%(v/v) Jeffamine ED-2001, 50 mM Tris–HCl pH 7.0 |
17% PEG 8000, 8% ethylene glycol, 0.1 M Tris–HCl pH 8.5 |
19% PEG 3350, 8% ethylene glycol, 0.1 M bis-Tris pH 6.0 |
1.1 M sodium malonate, 0.5%(v/v) Jeffamine ED-2001, 0.1 M HEPES pH 7.0 |
