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. 2020 Apr 29;76(Pt 5):209–215. doi: 10.1107/S2053230X20005427

Table 2. Crystallization.

Method Sitting-drop vapor diffusion
Plate type 48-well
Temperature (K) 293
Protein concentration (mg ml−1) 15
Buffer composition of protein solution 20 mM Tris HCl pH 8.0, 100 mM NaCl
Composition of reservoir solution 0.1 M Na HEPES pH 7.5, 20% PEG MME 2000
Volume and ratio of drop 1 µl, 1:1
Volume of reservoir (µl) 100