Fig. 1.

Antilymphoma activity of NK cells exposed to tofacitinib. After a 6-day culture with tofacitinib or control, NK cells were cocultured with B-NHL (Farage (EBV-positive) and SU-DHL-4 (EBV-negative)) cells. The percentage of lysis of Farage (a) and SU-DHL-4 (b) cells is shown (upper panel). The percentage of NK-mediated lysis was calculated as the percentage of dead target cells under specific conditions − the percentage of dead target cells cultured alone. The percentage of degranulating NK cells (CD107a-positive) is shown in the lower panel (c Farage cells, d SUDHL4 cells). IFNγ (e) and TNF (f) secretion was assessed by ELISA. The results are presented as mean and SD. Mann–Whitney t test. *p < 0.05, **p < 0.01, ***p < 0.001