Table 2.
Weights, Cd contents and isotope concentrations for yeast cells grown in culture media with 2 µmol L−1 CdCl2 (and δ114/110Cd = 0.36 ± 0.04‰)
| Construct | Dry weight (mg) | Cdtot (µg) | [Cd] (µg g−1) | δ114/110Cd (‰) | 2 s.d. (‰) | Δ114/110Cdtrans-ev (‰) |
|---|---|---|---|---|---|---|
| Empty vector | 19 | 2.62 | 138 | −0.18 | 0.09 | 0 |
| TcHMA2 | 23 | 4.32 | 188 | −0.20 | 0.06 | −0.02 |
| TcHMA3 | 4.4 | 0.231 | 52.5 | −0.42 | 0.04 | −0.24 |
| TcHMA3 SV | 21 | 0.537 | 25.6 | 0.03 | 0.02 | 0.21 |
| PtMt2b | 23 | 3.86 | 168 | −0.19 | 0.05 | −0.01 |
| TcNRAMP5 | 5.6 | 2.78 | 496 | −1.07 | 0.06 | −0.89 |
| TcNRAMP5 + TcHMA2 | 9.7 | 5.73 | 591 | −0.98 | 0.05 | −0.80 |
| TcNRAMP5 + TcHMA3 | 6.9 | 5.55 | 805 | −1.00 | 0.05 | −0.82 |
| TcNRAMP5 + PtMt2b | 9.7 | 5.94 | 613 | −0.94 | 0.05 | −0.76 |
Also shown is the apparent isotope fractionation Δ114/110Cdtrans-ev between transgenic yeast modified to express cacao proteins and the control (transformation with the empty vector).
s.d. standard deviation