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. 2020 Apr 30;11:2114. doi: 10.1038/s41467-020-16021-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Root colonisation of mutant and wild-type rice genotypes by R. irregularis. a AM colonisation levels of a cross between smax1 and the karrikin receptor mutant, dwarf 14-like (d14l), are quantified as a percentage (%) at 6 wpi with 300 spores. Dongjin and Nipponbare refer to the wild-type cultivars of respective mutant. F4 indicates the fourth generation progeny from the initial cross; d14/smax1(aa) indicates double homozygous alleles, whereas d14SMAX1 (AA, Aa) refers to homozygous d14l with either wild-type or heterozygous SMAX1 alleles. Each bar indicates the average percentage value of the respective fungal structure. Total total colonisation, H hyphopodia, IH intraradical hyphae, A arbuscules. Kruskal-Wallis test was performed, followed by post-hoc pairwise comparison using the Agricolae R package. Different letters represent significant difference (p-value <0.05). Degrees of freedom = 5, Dongjin wild-type (n = 4), smax1 (n = 4), Nipponbare wild-type (n = 4), d14l (n = 4), d14l/smax1 (F4, n = 5), d14l/SMAX1 (F4, n = 8), Total: χ² = 25.08, p = 0.0001; H: χ² = 17.84, p = 0.003; IH: χ² = 25.15, p = 0.0001; A: χ² = 25.67, p = 0.0001. b Micrographs of trypan blue stained roots for fungal structures in d14l/smax1 and their parental lines. Images represent roots from at least three independent plants of each genotype. Total total colonisation, H hyphopodia, IH intraradical hyphae, A arbuscules. Scale, 100 μm. c AM colonisation levels of crosses between smax1 and dwarf3 (d3). Dongjin and Shiokari refer to the wild-type cultivars of respective mutant. F4 indicates; d3/smax1(aa) indicates double homozygous alleles and d3/smax1 (AA, Aa) refers to homozygous d3 with either wild-type or heterozygous SMAX1 alleles. Degrees of freedom = 5, Dongjin wild-type (n = 5), smax1 (n = 5), Shiokari wild-type (n = 5), d3 (n = 4), d3/smax1 (F4, n = 5), d3/SMAX1 (F4, n = 8), Total: χ² = 26.01, p = 0.0001; H: χ² = 13.42, p = 0.02; IH: χ² = 25.65, p = 0.0001;A: χ² = 29.33, p = 2E0-5. d Micrographs of trypan blue stained roots for fungal structures in d3/smax1 and their parental lines.