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. 2020 Apr 30;11:2118. doi: 10.1038/s41467-020-15765-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Image of Paired-seq chip loaded with single cells and single beads, which were compartmented in water-in-gas droplets. b The occupation ratio of single particle in Paired-seq chip. Error bars, mean ± s.d., n = 4. c The statistical chart of bead and cell occupation ratio, pairing ratio and bead recovery ratio. Error bars, mean ± s.d., n = 3. d Single cell capture efficiency with different numbers of input cells. Error bars, mean ± s.d., n = 3. e Change of cell chamber fluorescence intensity indication mixing efficiency of TAMRA dye solution in bead chamber with PBS in cell chamber under conditions of free diffusion and pump driving. f Characterization of DNA hybridization on the surface of barcoded beads with target DNA and random DNA. Source data are provided as a Source Data file.