Fig. 1. Rad52 is involved in GCRs in the absence of Rad51.

a Two pathways of homologous recombination. Rad51, Rad52, and Rad54 are involved in Rad51-dependent recombination, whereas Rad52 but not Rad51 and Rad54 is required for single-strand annealing (SSA). b ChL^C is a derivative of Ch16, which is derived from fission yeast chr3. GCR and chromosome loss were detected by monitoring the genetic markers: LEU2, ura4⁺, and ade6⁺, which are present on ChL^C. c GCR and d chromosome loss rates were determined using the wild-type, rad51∆, rad52∆, and rad51∆ rad52∆ strains (TNF5369, 5411, 7493, and 7553, respectively). Each dot represents an independent experimental value obtained from an independent colony. Black lines indicate the median. Rates relative to the wild-type rate are shown on the top of each column. Statistical analyses between the wild-type and mutant strains and between the indicated pairs were performed using the two-tailed Mann–Whitney test. ****P < 0.0001. Source data for the graphs in c, d are available in Supplementary Data 1.