Table 1.
Primers for PCR detection of antimicrobial resistance determinants.
| Target | Primer sequence (5′–3′) | Annealing temperature (°C) | Amplicon size (bp) |
|---|---|---|---|
| QRDR of Topoisomerase genes | |||
| gyrAF | TCT CCG AGA TGG CCT GAA GC | 55 | 347 |
| gyrAR | TGC CGT CAT AGT TAT CCA CG | ||
| gyrBF | CAA ACT GGC GGA CTG TCA GG | 55 | 345 |
| gyrBR | TTC CGG CAT CTG ACG ATA GA | ||
| parCF | CTA TGC GAT GTC AGA GCT GC | 55 | 275 |
| parCR | TGA CCG AGT TCG CTT AAC AG | ||
| parEF | GAC CGA GCT GTT CCT TGT GG | 60 | 492 |
| parER | GCG TAA CTG CAT CGG GTT CA | ||
| PMQR | |||
| qnrAF | GAG GAT TTC TCA CGC CAG GA | 60 | 575 |
| qnrAR | TGC CAG GCA CAG ATC TTG AC | ||
| qnrB1F | GAT CGT GAA AGC CAG AAA GG | 55 | 468 |
| qnrB1R | ACG ATG CCT GGT AGT TGT CC | ||
| qnrB2F | GTT GGC GAA AAA ATT GAC AGA A | 57 | 451 |
| qnrB2R | TTT GCA AGG CGT CAA ACT GG | ||
| qnrCF | GGG TTG TAC ATT TAT TGA ATC | 47 | 446 |
| qnrCR | TCC ACT TTA CGA GGT TCT | ||
| qnrDF | CGA GAT CAA TTT ACG GGG AAT A | 60 | 581 |
| qnrDR | AAC AAG CTG AAG CGC CTG | ||
| qnrSF-1 | GGA AAC CTA CAA TCA TAC ATA TCG GC | 55 | 530 |
| qnrSR-1 | TAA ATT GGC ACC CTG TAG GC | ||
| qnrSF-2 | ATG GAA ACC TAC CGT CAC AC | 55 | 638 |
| qnrSR-2 | ATA CCC AAC GCT TCG AGA AG | ||
| aac(6′)-ib-crF | GCA ACG CAA AAA CAA AGT TAG G | 47 | 560 |
| aac(6′)-ib-crR | GTG TTT GAA CCA TGT ACA | ||
| qepAF | GCA GGT CCA GCA GCG GGT AG | 63 | 217 |
| qepAR | CTT CCT GCC CGA GTA TCG TG | ||