Fig. 7.
a Interaction between PbMC1a/1b and PbRD21 verified by Y2H analysis. Selective growth of the Y2H system was performed by using the X-gal activity assay. Yeast cells were cotransformed with pGAD-PbRD21 + pGBKT7 and pGBK-PbMC1a/1b + pGADT7. pGADT7-T + pGBKT7-Lam was used as a negative control, and pGADT7-T + pGBKT7-53 was used as a positive control. b BiFC assay using Nicotiana benthamiana. The negative controls were PbMC1a/1b-YFPN + YFPC and PbRD21-YFPN + YFPC. All images were taken by confocal microscopy. Bar: 40 μm. c Interaction assay via GST pull-down analysis. PbMC1a/1b and PbRD21 fused with GST and HIS tags were mixed and passed through a glutathione column (binding GST tag). After elution with pull-down binding buffer, the samples were separated by SDS-PAGE. GST alone was used as the negative control