Figure 2.

Myogenesis in the early bovine embryo. Day 23 and 45 bovine embryos were fixed and immunostained for key myogenic muscle proteins. MyHC (A) expression localized to the somites (S) and heart (H) of a day 23 embryo. Cryosections through the rostral somites were immunostained for Pax7 (green) and counterstained with phalloidin (red) (B). Coronal sections through a day 45 embryo stained with Hoechst 33324 for nuclei (C). The red box denotes a forelimb with magnification in the remaining panels. Pax7 and Myf5 co-localize to distinct limb myoblast subpopulations. Bar represents 1 mm or 50 µm.