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. 2020 Apr 24;11:323. doi: 10.3389/fgene.2020.00323

FIGURE 1.

FIGURE 1

Streptococcus pneumoniae D39 planktonic and biofilm growth in metal ion-free BHI medium. (A) Time-dependent planktonic growth from 0 to 48 h with different concentrations of ASD. Growth was detected by measuring optical density at 600 nm. (B) Planktonic growth detected by resazurin staining after 48 h incubation. (C) Quantification of in vitro biofilm biomass grown for 48 h using a CV-microplate assay. (D) Colony-forming unit (cfu) counts of in vitro biofilms grown for 48 h. (E) Quantification of in vitro biofilm growth using resazurin staining grown for 48 h. (F) Quantification of in vitro biofilm growth of Pseudomonas aeruginosa, MRSA, and E. coli grown for 48 h using resazurin staining. Error bars are the standard deviation from the mean. Statistical significance was calculated using the Student’s t-test, and P-value < 0.05 was considered significant (∗P < 0.05).