TABLE 1.
Serial no. | Gene name | Primer sequence | Amplicon |
1 | 16s | 5′-AACCAAGTAACTTTGAAAGAAGAC-3′ | 126 bp |
5′-AAATTTAGAATCGTGGAATTTTT-3′ | |||
2 | luxS | 5′-ACATCATCTCCAATTATGATATTC-3′ | 257 bp |
5′-GACATCTTCCCAAGTAGTAGTTTC-3′ | |||
3 | comA | GAGACGCGAGCCATTAAGG | 156 bp |
GGGATCTGGATCGGCAATATGA | |||
4 | comB | 5′-GAACCCAGTCGTATCCTTGC-3′ | 95 bp |
5′-TCCCCCTTCTTAACCAGCTT-3′ | |||
5 | ciaR | GATGTTATGCAGGTATTTGATG | 157 bp |
TAATCAGAACTGGTGTCGTAAT | |||
6 | ply | TGAGACTAAGGTTACAGCTTACAG | 225 bp |
CTAATTTTGACAGAGAGATTACGA | |||
7 | lytA | CGTCCCAGGCACCATTATCA | 95 bp |
CTGGCGGAAAGACCCAGAAT |