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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Pain. 2019 Sep;160(9):2161–2171. doi: 10.1097/j.pain.0000000000001583

Figure 1. Experimental Design and Setup.

Figure 1.

Infusion and Timing of Treatments. (A) A catheter was surgically implanted through a cranial window made adjacent to the lambda suture. The loose end of the catheter was routed subcutaneously exiting the skin in the medial facial region to allow for either IS or SIF dural application. (B) Experimental timeline: IS or SIF was applied to the dura mater of each animal and then returned to their home-cage for forty five minutes. Next, a fifteen-minute MRI setup was then performed while the animal was under 2% isoflurane. During this time, the animals were placed in the imaging holder and a tail-vein catheter was inserted. After setup, the animal was placed in the MRI to calibrate the magnet and acquire structural (T1) scans. Functional MRI scanning started forty-five minutes after the completion of the pre-MRI setup to mitigate any residual effects of isoflurane. Migraine treatment was captured during a fifteen-minute fMRI scan with infusion of sumatriptan (1mg/kg) and then naproxen (1mg/kg) independently over the course of one minute, to prevent potential precipitation of the drug if mixed.