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. 2020 May;62(5):645–656. doi: 10.1165/rcmb.2019-0310OC

Figure 7.

Figure 7.

Role of SLK in the Plk1-paxillin pathway on stimulation with histamine and 5-HT. (A) Extracts of unstimulated or stimulated HASM cells treated with Ctrl or SLK siRNA were immunoblotted with antibodies against phospho-Plk1 (Thr-210) and total Plk1. Data are mean values of experiments from four batches of cell culture from three donors. Error bars indicate SD. (B and C) Paxillin phosphorylation at Ser-272 and F/G-actin ratios on stimulation with histamine or 5-HT are reduced by SLK KD. Data are mean values of experiments from four batches of cell culture from three donors. Error bars indicate SD. (D) Histamine- or 5-HT–stimulated myosin light chain phosphorylation at Ser-19 is not affected by SLK KD. Data are mean values of experiments from four batches of cell culture from three donors. Error bars indicate SD. *P < 0.05 and **P < 0.01. Two-way ANOVA was used for statistical analysis.