Figure 6.

PI3K inhibition with LY decreased goblet cell hyperplasia in Ad-TGFβ–exposed CF, but not non-CF, mice. (A) Periodic acid–Schiff–stained, mucin-containing goblet cells (arrows) were more numerous in Ad-TGFβ–exposed CF mice treated with vehicle. Scale bar: 50 μm. (B) The percentage of goblet cells around airways was significantly increased in vehicle-treated CF mice after Ad-TGFβ exposure, but this goblet cell hyperplasia was decreased by LY treatment. Percent goblet cells was not changed by PI3K inhibition in Ad-TGFβ–exposed non-CF mice. *P < 0.05 by one-way ANOVA with Tukey’s post hoc analysis. Data are presented as mean ± SD.