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. 2020 Mar 25;37(1):31–38. doi: 10.5511/plantbiotechnology.19.1209b

Figure 1. Purification and characterization of α-amylase: (A) Elution profile of purification using epoxy-activated Sepharose 6B linked with β-cyclodextrin (β-CD) for affinity chromatography. The fractions were analyzed enzyme activity (■) and protein concentration at A280 nm (●). (B) Electrophoresis pattern and amylase activity. Molecular weight markers (lane a) and purified enzyme (lane b) using SDS-PAGE, amylase activity by Zymographic method (lane c). (C) Digested peptides which showed match with α-amylase sequence from plant α-amylases.

Figure 1. Purification and characterization of α-amylase: (A) Elution profile of purification using epoxy-activated Sepharose 6B linked with β-cyclodextrin (β-CD) for affinity chromatography. The fractions were analyzed enzyme activity (■) and protein concentration at A280 nm (●). (B) Electrophoresis pattern and amylase activity. Molecular weight markers (lane a) and purified enzyme (lane b) using SDS-PAGE, amylase activity by Zymographic method (lane c). (C) Digested peptides which showed match with α-amylase sequence from plant α-amylases.