Table 3. Effect of metal ions and chemical reagents on the α-amylase activity.
| Different additives | Concentration | Relative activity (%) |
|---|---|---|
| Control (Non-component) | — | 100±0.02i |
| Metal ions | ||
| NaCl | 5 mM | 95.40±1.00gh |
| KCl | 5 mM | 90.71±0.81f |
| CaCl2 | 5 mM | 89.47±0.97j |
| MgCl2 | 5 mM | 82.04±1.21e |
| ZnCl2 | 5 mM | 81.57±0.64e |
| BaCl2 | 5 mM | 90.48±0.33f |
| FeCl2 | 5 mM | 78.88±0.48e |
| CdCl2 | 5 mM | 74.56±1.74d |
| CoCl2 | 5 mM | 107.84±1.14m |
| CuCl2 | 5 mM | 35.77±0.36b |
| NiCl2 | 5 mM | 53.27±0.23c |
| Chemical reagents | ||
| EDTA | 10 mM | 0.00±0.00a |
| β-mercaptoethanol | 10 mM | 117.65±2.58l |
| SDS | 5% | 0.00±0.00a |
| Triton X-100 | 5% | 95.17±0.54gh |
| Tween-20 | 5% | 114.10±1.04k |
| Methanol | 10% | 84.06±1.56fg |
| Ethanol | 10% | 82.87±0.69h |
| Acetone | 10% | 117.64±2.36l |
| Hexane | 10% | 92.80±0.35fg |
Mean value followed by different letters differs significantly (p≤0.05).