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. 2020 Mar 25;37(1):39–46. doi: 10.5511/plantbiotechnology.19.1212a

Figure 4. GFP fluorescence and genotyping of hairy roots. Analysis of hairy roots generated from stem explants, which had been infected with A13 harboring pBI121_GFPh. (A) Generated hairy root (line 2) showing GFP fluorescence. Bright field (left) and GFP fluorescence (right) images were taken at the same field of view. Scale bars, 2 mm. (B) Confirmation of transgene integration by genotyping PCR. Genomic DNA extracted from hairy roots was subjected to PCR amplification of the transgene GFPh and the endogenous gene Actin. Controls consisted of genomic DNA extracted from hairy roots generated with wild-type A13 (N, no plasmid) and the plasmid pBI121_GFPh (P). ++, very strong fluorescence; +, strong fluorescence; −, no fluorescence.

Figure 4. GFP fluorescence and genotyping of hairy roots. Analysis of hairy roots generated from stem explants, which had been infected with A13 harboring pBI121_GFPh. (A) Generated hairy root (line 2) showing GFP fluorescence. Bright field (left) and GFP fluorescence (right) images were taken at the same field of view. Scale bars, 2 mm. (B) Confirmation of transgene integration by genotyping PCR. Genomic DNA extracted from hairy roots was subjected to PCR amplification of the transgene GFPh and the endogenous gene Actin. Controls consisted of genomic DNA extracted from hairy roots generated with wild-type A13 (N, no plasmid) and the plasmid pBI121_GFPh (P). ++, very strong fluorescence; +, strong fluorescence; −, no fluorescence.