Skip to main content
. 2020 Mar 25;37(1):83–88. doi: 10.5511/plantbiotechnology.19.1227a

Figure 1. Construction of the CMV vector for VIGS. (A) Schematic representation of the CMV vector A1, whose backbone is RNA2 encoding the 2a and 2b genes. The spinach genes (PDS and DFR) were cloned between the StuI and MluI site in the antisense orientation. (B) Symptoms on the upper leaves of spinach (‘All Right’) infected with the original vectors of Y1A1Y3 (left) and L1A1L3 (right).

Figure 1. Construction of the CMV vector for VIGS. (A) Schematic representation of the CMV vector A1, whose backbone is RNA2 encoding the 2a and 2b genes. The spinach genes (PDS and DFR) were cloned between the StuI and MluI site in the antisense orientation. (B) Symptoms on the upper leaves of spinach (‘All Right’) infected with the original vectors of Y1A1Y3 (left) and L1A1L3 (right).