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. 2020 Mar 25;37(1):9–14. doi: 10.5511/plantbiotechnology.19.1120a

Figure 1. Callus induction from kenaf seed, hypocotyl, and cotyledon. (A–C) Callus induction from kenaf seeds. Kenaf seeds (A) were sterilized and then were placed on CIM (B). Calli were induced from the seeds by cultivating on CIM for one week (C). (D–F) Callus induction from the kenaf hypocotyl. Hypocotyls from 10-day-old seedlings (D) were cut and placed vertically on CIM (E). Calli were induced from the fragmented hypocotyls by cultivating on CIM for one week (F). (G–I) Callus induction from the kenaf cotyledon. Cotyledons from 10-day-old seedlings (G) were cut into small pieces and then were cultivated on CIM (H). Calli were induced from cotyledon pieces by cultivating on CIM for three weeks (I). Bars=10 mm.

Figure 1. Callus induction from kenaf seed, hypocotyl, and cotyledon. (A–C) Callus induction from kenaf seeds. Kenaf seeds (A) were sterilized and then were placed on CIM (B). Calli were induced from the seeds by cultivating on CIM for one week (C). (D–F) Callus induction from the kenaf hypocotyl. Hypocotyls from 10-day-old seedlings (D) were cut and placed vertically on CIM (E). Calli were induced from the fragmented hypocotyls by cultivating on CIM for one week (F). (G–I) Callus induction from the kenaf cotyledon. Cotyledons from 10-day-old seedlings (G) were cut into small pieces and then were cultivated on CIM (H). Calli were induced from cotyledon pieces by cultivating on CIM for three weeks (I). Bars=10 mm.