Table 1. Resulting amplifications by verifying and genotyping P. larvae strains by multiplexed qPCR with different targets by their “final call (ΔRn)”. –, no amplification of the taget; +, amplification of the target.
| P. larvae strain | 16S rRNA | ItuC | plx1.3 |
|---|---|---|---|
| Paenibacillus larvae ERIC I | |||
| DSM 7030 | + | + | + |
| Isolate 5 | + | + | + |
| Isolate 9 | + | + | + |
| Isolate 11 | + | + | + |
| Isolate 15 | + | + | + |
| Isolate 22 | + | + | + |
| Isolate 24 | + | + | + |
| Isolate 25 | + | + | + |
| Isolate 26 | + | + | + |
| Isolate 29 | + | + | + |
| Isolate 145 | + | + | + |
| Isolate 146 | + | + | + |
| Isolate 148 | + | + | + |
| Isolate 153 | + | + | + |
| Isolate 155 | + | + | + |
| Isolate 157 | + | + | + |
| Isolate 159 | + | + | + |
| Isolate 160 | + | + | + |
| Isolate 162 | + | + | + |
| Isolate 174 | + | + | + |
| Paenibacillus larvae ERIC II | |||
| DSM 16116 | + | + | − |
| DSM 25430 | + | + | − |
| DSM 17237 (RSK16) | + | + | − |
| Isolate 1 | + | + | − |
| Isolate 3 | + | + | − |
| Isolate 6 | + | + | − |
| Isolate 7 | + | + | − |
| Isolate 17 | + | + | − |
| Isolate 20 | + | + | − |
| Isolate 23 | + | + | − |
| Isolate 27 | + | + | − |
| Isolate 28 | + | + | − |
| Isolate 135 | + | + | − |
| Isolate 137 | + | + | − |
| Isolate 144 | + | + | − |
| Isolate 146 | + | + | − |
| Isolate 152 | + | + | − |
| Isolate 156 | + | + | − |
| Isolate 158 | + | + | − |
| Isolate 161 | + | + | − |
| Isolate 168 | + | + | − |
| Paenibacillus alvei (DSM 29) | − | − | − |
| No Template Control (NTC) | − | − | − |