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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Am J Transplant. 2019 Jul 24;20(1):75–87. doi: 10.1111/ajt.15517

Figure 6. Circulating Tfh cells predict breakthrough alloantibodies despite immunosuppression.

Figure 6.

Naïve B6 mice were transplanted with BALB/c skin grafts and treated with tacrolimus (FK-506), CTLA-4-Ig, anti-CD28 dAb, or left untreated. BALB/c-grafted mice were sacrificed 10 days post-transplant for PBMC and graft-dLN analysis and serially bled for DSA assessment. (A) Representative flow cytometric plots (gated on CD4+Foxp3− T cells) displaying the frequencies of CXCR5+ cTfh cells. (B) Summary data of the frequencies and numbers of cTfh cells (n=5 per group). (C) Representative flow plots (gated on CD4+Foxp3−CXCR5+ T cells) displaying the frequencies of ICOS+PD-1+ cTfh cells. (D) Summary data of the frequencies and numbers of ICOS+PD-1+ cTfh cells (n=5 per group). Summary data of the frequencies and numbers of graft-dLN (E) Tfh and (F) GC B cells (n=5 per group). (G) Summary data of anti-donor IgG formation in treated or untreated BALB/c-grafted mice over time (n=5 per group). Summary data represent mean (SE) and are representative of three independent experiments with a total of 15 mice per group. *p < 0.05, **p < 0.01, ***p< 0.001.