Table 1.
Target gene | Primers (from 5′ to 3′) | Length |
---|---|---|
iNOS2 | Forward 5′- GAAGTGGTATGCTCTGCCTGCTG-3′ Reverse 5′- GTCTCGCACTCCAATCTCTGTTCC-3′ |
115 |
TNF-α | Forward 5′- TGATCGTGACACGTCTCTGC-3′ Reverse 5′- CAACCAGCTATGCACCCCAG-3′ |
88 |
IL-6 | Forward 5′- TTCACCGTGTGCGAGAACAGC-3′ Reverse 5′- CAGCCGTCCTCCTCCGTCAC-3′ |
80 |
IL-1β | Forward 5′- AGCAGCCTCAGCGAAGAGACC-3′ Reverse 5′- GTCCACTGTGGTGTGCTCAGAATC-3′ |
90 |
Bax | Forward 5′- ACTCTGCTGCTGCTCTCCTCTC-3′ Reverse 5′- ATCCACGCAGTGCCAGATGTAATC-3′ |
174 |
Caspase-3 | Forward 5′- TACCGGACTGTCATCTCGTTCAGG-3′ Reverse 5′- ACTGCTTCGCTTGCTGTGATCTTC-3′ |
166 |
Caspase-8 | Forward 5′- GGAAGCAGTGCCAGAACTCAGAAG-3′ Reverse 5′- TTGTTGTGGTCCATGCACCGATAG-3′ |
174 |
Caspase-9 | Forward 5′- CCGAAGGAGCAAGCACGACAG-3′ Reverse 5′- CATCTAGCATGTCAGCCAGGTCAC-3′ |
121 |
P53 | Forward 5′- GGAGATGGAACCATTGCTGGAACC-3′ Reverse 5′- GCTCCTGCCAGTTGCTGTGATC-3′ |
113 |
Bcl2 | Forward 5′- GAGTTCGGCGGCGTGATGTG-3′ Reverse 5′- TTCAGGTACTCGGTCATCCAGGTG-3′ |
92 |
Cytochrome C | Forward 5′- CCTAATCGCCGTGGCCTTCTTAAC-3′ Reverse 5′- GGAGGAGGTAGATGGTCGGATTGG-3′ |
163 |
β-actin | Forward 5′- CAACACAGTGCTGTCTGGTGGTAC-3′ Reverse 5′- CTCCTGCTTGCTGATCCACATCTG-3′ |
199 |