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. 2020 Apr 24;7:225. doi: 10.3389/fvets.2020.00225

Table 1.

List of primers used in RT-PCR.

Target gene Primers (from 5 to 3) Length
iNOS2 Forward 5′- GAAGTGGTATGCTCTGCCTGCTG-3′
Reverse 5′- GTCTCGCACTCCAATCTCTGTTCC-3′
115
TNF-α Forward 5′- TGATCGTGACACGTCTCTGC-3′
Reverse 5′- CAACCAGCTATGCACCCCAG-3′
88
IL-6 Forward 5′- TTCACCGTGTGCGAGAACAGC-3′
Reverse 5′- CAGCCGTCCTCCTCCGTCAC-3′
80
IL-1β Forward 5′- AGCAGCCTCAGCGAAGAGACC-3′
Reverse 5′- GTCCACTGTGGTGTGCTCAGAATC-3′
90
Bax Forward 5′- ACTCTGCTGCTGCTCTCCTCTC-3′
Reverse 5′- ATCCACGCAGTGCCAGATGTAATC-3′
174
Caspase-3 Forward 5′- TACCGGACTGTCATCTCGTTCAGG-3′
Reverse 5′- ACTGCTTCGCTTGCTGTGATCTTC-3′
166
Caspase-8 Forward 5′- GGAAGCAGTGCCAGAACTCAGAAG-3′
Reverse 5′- TTGTTGTGGTCCATGCACCGATAG-3′
174
Caspase-9 Forward 5′- CCGAAGGAGCAAGCACGACAG-3′
Reverse 5′- CATCTAGCATGTCAGCCAGGTCAC-3′
121
P53 Forward 5′- GGAGATGGAACCATTGCTGGAACC-3′
Reverse 5′- GCTCCTGCCAGTTGCTGTGATC-3′
113
Bcl2 Forward 5′- GAGTTCGGCGGCGTGATGTG-3′
Reverse 5′- TTCAGGTACTCGGTCATCCAGGTG-3′
92
Cytochrome C Forward 5′- CCTAATCGCCGTGGCCTTCTTAAC-3′
Reverse 5′- GGAGGAGGTAGATGGTCGGATTGG-3′
163
β-actin Forward 5′- CAACACAGTGCTGTCTGGTGGTAC-3′
Reverse 5′- CTCCTGCTTGCTGATCCACATCTG-3′
199