Fig. 12. SsnB downregulates STAT3 phosphorylation, decreases stellate cell activation and connective tissue growth factor in vitro.
A. Western blot analysis of phospho STAT3, α-SMA, CTGF and β-actin protein levels of Control (untreated), LPS-treated, LPS+SsnB10 (10 μM), and LPS+SsnB100 (100 μM) treated human immortalized hepatic stellate cells (LX2). B. Morphometric analysis of western blot where the bar diagram represents the level of pSTAT3, α-SMA, and CTGF normalized against β-actin of respective samples. (*) P < 0.05 is considered statistically significant.