Fig. 5.
In vivo glucose and mitochondrial oxidative metabolism in WD-mediated NASH and the effects of VitE treatment. Rates for enzyme-catalyzed reactions of the CAC, anaplerosis, cataplerosis, and glucose production (A–I) were estimated using metabolic flux analysis in 20 h-fasted mice fed chow, chow+VitE, WD for 8 weeks, WD+VitE for 8 weeks, WD for 20 weeks, and WD+VitE for 20 weeks. Individual fluxes were regressed using the enrichment of mass isotopomers of plasma glucose obtained from the artery of conscious, unstressed mice during the isotopic steady state. Data are presented as mean ± SEM (n = 8–10, shown as white circles). *P < 0.05 versus chow, #P < 0.05 versus WD for 8 weeks, VitE effects are indicated by brackets on each graph. VGK, gluconeogenesis from glycerol.