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. 2020 Mar 26;61(5):734–745. doi: 10.1194/jlr.RA119000294

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Fig. 2. — Effects of Slc43a3 knockdown and overexpression on genes involved in lipid metabolism. A: Lipid accumulation in differentiated OP9 cells transfected with scrambled siRNA (control), Slc43a3 siRNA, pcDNA6 (control), or pEZ-M51-Slc43a3-His clone. OP9 cells were transfected with the scrambled siRNA (control), Slc43a3 siRNA, pcDNA6 (control), or pEZ-M51-Slc43a3-His clone and differentiated for 5 days. Bodipy 493/503 (1 nM) was added in the media before fluorescent microscopy. Results are representative of three independent experiments. Scale bar 200 microns. B: Western blot of Slc43a3 in OP9 cells transfected with scrambled siRNA (control), Slc43a3 siRNA, pcDNA6 (control), or pEZ-M51-Slc43a3-His clone. C: Effects of knockdown and overexpression of Slc43a3 in OP9 cells on genes involved in lipid metabolism. Results are representative of two to three independent experiments. D: Effects of knockdown and overexpression of Slc43a3 in OP9 cells on genes involved in esterification. Results are representative of two independent experiments, each with n = 4–6. *P < 0.05.