Fig. 3.

Effects of Slc43a3 knockdown and overexpression on FA transport. A: Effects of knockdown and overexpression of Slc43a3 in OP9 cells on FA uptake. Results are representative of two to three independent experiments. Slc43a3 knockdown, n = 22; Slc43a3 overexpression, n = 12; Control combined, n = 36. *P < 0.05, **P < 0.01, ***P < 0.001. B: Effects of knockdown of Slc43a3 in OP9 cells on FA efflux. Transfected OP9 cells were loaded with fluorescent lipid BODIPY C1-C12 bound to 1% BSA, and FA efflux assayed following the quencher-based FA efflux assay. Results are representative of three to four independent experiments with replication of nine in each treatment. The cumulative FA efflux was calculated for the first 5 min of the reaction. *P < 0.05, **P < 0.01. C: Effects of knockdown of Slc43a3 in OP9 cells on FA efflux using ¹⁴C-palmitic acid. Transfected and differentiated OP9 cells were loaded with 20 μM ¹⁴C-palmitic acid. Cells were then stimulated either with 20 μM forskolin and 1 mM isobutyl-methylxanthine or 100 nM isoproterenol for lipolysis. Media were collected after 60 min for measurement of FA radioactivity and glycerol released (D). *P < 0.05, **P < 0.01.