Fig. 1.

Temperature dependence of phase separation and nanodomain formation in RBL-2H3 GPMVs: effect of DTT concentration. A: Phase separation assayed by fluorescence microscopy. B: Nanodomain formation assayed by FRET. Samples for microscopy contained FAST DiO. Samples for FRET contained DPH and ODRB. GPMVs prepared with DTT and 24 mM PFA. F/Fo values are normalized to 1 at the highest temperature at which measurements were made (44°C). Unnormalized F/Fo values at 44°C for this and later figures are shown in supplemental Table S1. Symbols: no DTT (diamonds); 0.5 mM DTT (squares); 1 mM DTT (triangles); 2 mM DTT (circles); 5 mM DTT (crosses). In this and the following figures, mean values and standard deviations (up bars only) for n = 3 are shown unless otherwise noted.