Fig. 3.

Effect of sterol exchange in cells upon phase separation and nanodomain formation in RBL-2H3 GPMVs. A, C: Phase separation assayed by fluorescence microscopy. Symbols: untreated (diamonds); 7-dehydrocholesterol (filled squares); coprostanol (filled triangles); desmosterol (circles); epicholesterol (crosses); lanosterol (open squares); 4-cholesten-3-one (open triangles). B, D: Nanodomain formation assayed by FRET. Symbols: untreated (diamonds); cholesterol-depleted (filled squares); exchange with 0.1 mM cholesterol (filled triangles); exchange with 0.4 mM cholesterol (circles). Samples prepared using 2 mM DTT and 24 mM PFA (n = 3; except n = 9–10 for the untreated samples).