TABLE 1.

T mid and T end values for large-scale phase separation and nanodomain formation for RBL-2H3 GPMVs prepared with 24 mM at different DTT concentrations

DTT Concentration (mM)	Phase Separation		Nanodomains
	T mid	T end	T end
0	0.5 ± 2.0	5.4 ± 2.4	26.7 ± 2.1
0.5	10.6 ± 2.5	15.1 ± 3.9	31.4 ± 2.5
1	12.5 ± 2.0	19.2 ± 3.6	34.6 ± 1.4
2	14.8 ± 0.7	22.7 ± 0.4	38.2 ± 1.1
5	19.1 ± 0.9	24.7 ± 5.0	37.4 ± 1.5

The data were fitted to a sigmoidal curve of percent GPMVs with domains versus temperature, assuming the limit at extremely low temperature would equal 100% of vesicles containing domains. For phase separation, T mid was defined as temperature at which 50% of the vesicles would have domains, and T end the temperature above which less than 1% of vesicles contain domains. For nanodomains, T end was defined as the temperature at which F/Fo was a minimum (see Methods). Mean values and standard deviations from the results from three preparations are shown.