TABLE 2.
T mid and T end values for large-scale phase separation and nanodomain formation for RBL-2H3 GPMVs prepared using 2 mM DTT and 24 mm PFA after different substitutions or additions
| Substitution | Phase Separation | Nanodomains | |
| T mid | T end | T end | |
| Untreated | 12.2 ± 1.3 | 18.6 ± 2.1 | 38.5 ± 2.4 |
| POPC | 4.4 ± 1.9 | 7.6 ± 1.6 | 33.3 ± 2.1 |
| SM | 20.1 ± 1.3 | 25.9 ± 2.4 | 42.5 ± 1.1 |
| Coprostanol | 2.6 ± 1.1 | 7.6 ± 3.3 | 32.9 ± 2.1 |
| 4-Cholesten-3-one | −1.3 ± 0.8 | 11.1 ± 1.1 | 32.9 ± 2.7 |
| Desmosterol | 10.1 ± 1.5 | 14.9 ± 2.1 | 37.1 ± 3.5 |
| Epicholesterol | 10.9 ± 1.5 | 18.0 ± 2.7 | 31.4 ± 0.2 |
| Lanosterol | 11.5 ± 1.8 | 18.5 ± 3.9 | 34.5 ± 1.8 |
| 7-Dehydrocholesterol | 21.9 ± 1.5 | 25.7 ± 1.8 | 44.1 ± 2.9 |
| Cholesterol depleted | 2.9 ± 2.5 | 17.8 ± 1.8 | 30.3 ± 1.4 |
| Cholesterol loaded (0.1 mM) | 16.8 ± 1.2 | 20.5 ± 3.6 | 37.1 ± 1.3 |
| Cholesterol loaded (0.4 mM) | 19.7 ± 2.8 | 24.1 ± 2.4 | 38.8 ± 2.2 |
Mean values and standard deviations from the results from three preparations are shown, except for untreated samples (samples without any lipid substitutions) for which n = 9.