Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 24;11:511. doi: 10.3389/fphar.2020.00511

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Udawatte, Kang, Wang, Arumugam and Seneviratne

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Kim-1 and CLU immunoreactivity in the kidneys of SD rats treated with a single intravenous dose of 15mg/kg/day BW for 5 days. In Kim-1 immunoreactivity was shown in, (A) vehicle, (B) AmB and (C) SM21. Only occasional Kim-1-positive tubules were detected in SM21 (blue arrow in C) kidneys. Kim-1 was prominently detected translocated to an apical (luminal) membrane of convoluted portion of the proximal tubules in AmB group. In CLU immunoreactivity was shown in, (D) Vehicle (E) AmB, (F) SM21. In AmB group, high expression of CLU observed over almost all different tubules. Positive pixel analysis identified a low increase in immunoreactivity in rats dosed with SM21 group compared to control. However, this expression is comparatively lower than AmB treatment. No immunoreactivity of Kim-1 and CLU were detected in vehicle group. IHC profiler was used to assign an automated score to the respective IHC images by computerized pixel profiling based on colour deconvolution acquired on Image J. Kim-1, Kidney injury molecule-1; CLU, Clusterin; SD, Sprague Dawley; IHC, Immunohistochemistry.