Figure 9.

There was a significant level of correlation between tissue (x-axis) and urinary (y-axis), (A) Kim-1 and (B) Clusterin from individual animals on the day 5. Vehicle, SM21 and SM21 analogue treated rats had little to no urinary expression of protein nor protein immunoreactivity within the kidney, whereas AmB-treated rats had higher levels of both urinary protein expression and immunoreactivity observed within the kidney. The line of best following linear regression of Kim-1 and clusterin are shown (R² = 0.782) and (R² = 0.724), respectively. The IHC optical density score calculation (Image J) of the each zone is assigned as 4 for the high positive zone, 3 for the positive zone, 2 for the low positive zone, and 1 for the negative zone. IHC, immunohistochemistry; Kim-1, Kidney Injury molecule-1; SMA, SM21 Analogue.